Resolution of 2-bromo-arylacetic acid ester by Yarrowia lipolytica lipase in water/supercritical CO2 two-phase systems
نویسندگان
چکیده
منابع مشابه
Two-step synthesis of fatty acid ethyl ester from soybean oil catalyzed by Yarrowia lipolytica lipase
BACKGROUND Enzymatic biodiesel production by transesterification in solvent media has been investigated intensively, but glycerol, as a by-product, could block the immobilized enzyme and excess n-hexane, as a solution aid, would reduce the productivity of the enzyme. Esterification, a solvent-free and no-glycerol-release system for biodiesel production, has been developed, and two-step catalysi...
متن کاملThe production of Yarrowia lipolytica lipase powder by improved spray-drying method
Lipase is used in the production of foods, flavor enhancers, detergents, cosmetics and pharmaceuticals. A common impediment to the production of commercial enzymes is their low-stability aqueous solutions. In this study, the downstream process was investigated to obtain a stable spray-dried lipase powder of Yarrowia lipolytica. The enzyme solution samples were supplemented with different concen...
متن کاملYarrowia lipolytica lipase production enhanced by increased air pressure.
AIMS To study the cellular growth and morphology of Yarrowia lipolytica W29 and its lipase and protease production under increased air pressures. METHODS AND RESULTS Batch cultures of the yeast were conducted in a pressurized bioreactor at 4 and 8 bar of air pressure and the cellular behaviour was compared with cultures at atmospheric pressure. No inhibition of cellular growth was observed by...
متن کاملImproving the thermostability of lipase Lip2 from Yarrowia lipolytica.
Yarrowia lipolytica lipase Lip2 (YlLip2) is a highly versatile biocatalyst. However, its practical use is often hampered by its low stability. Here three complementary protein engineering strategies were used to improve the thermostability of this enzyme. The first strategy was error-prone PCR based directed evolution, which resulted in a YlLip2 variant with a 2.5-fold longer half-life of therm...
متن کاملCharacterization of an Extracellular lipase from Yarrowia lipolytica
Lipases from different sources have distinct properties, such as specificity, stability and optimal operational conditions. Moreover, different conditions of lipase production may alter these enzyme properties. Therefore, the aims of this study were related to the evaluation of lipase activity of Yarrowia lipolytica IMUFRJ 50682 (isolated from Baía de Guanabara, Brazil) produced under submerged...
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ژورنال
عنوان ژورنال: The Journal of Supercritical Fluids
سال: 2017
ISSN: 0896-8446
DOI: 10.1016/j.supflu.2016.11.012